This workflow uses the decoupler tool in Galaxy to generate pseudobulk counts from an annotated AnnData file obtained from scRNA-seq analysis. Following the pseudobulk step, differential expression genes (DEG) are calculated using the edgeR tool. The workflow also includes data sanitation steps to ensure smooth operation of edgeR and minimizing potential issues. Additionally, a Volcano plot tool is used to visualize the results after the DEG analysis. ## Associated Tutorial This workflows is part of the tutorial [Pseudobulk Analysis with Decoupler and EdgeR](https://training.galaxyproject.org/training-material/topics/single-cell/tutorials/pseudobulk-analysis/tutorial.html), available in the [GTN](https://training.galaxyproject.org) ## Features * Includes [Galaxy Workflow Tests](https://training.galaxyproject.org/training-material/faqs/gtn/workflow_run_test.html) * Includes a [Galaxy Workflow Report](https://training.galaxyproject.org/training-material/faqs/galaxy/workflows_report_view.html) ## Thanks to... **Workflow Author(s)**: Diana Chiang Jurado, Pavankumar Videm, Pablo Moreno **Tutorial Author(s)**: [Diana Chiang Jurado](https://training.galaxyproject.org/training-material/hall-of-fame/dianichj/) **Tutorial Contributor(s)**: [Pavankumar Videm](https://training.galaxyproject.org/training-material/hall-of-fame/pavanvidem/), [Björn Grüning](https://training.galaxyproject.org/training-material/hall-of-fame/bgruening/), [Saskia Hiltemann](https://training.galaxyproject.org/training-material/hall-of-fame/shiltemann/), [Diana Chiang Jurado](https://training.galaxyproject.org/training-material/hall-of-fame/dianichj/) [](https://training.galaxyproject.org/training-material/)