# Summary This notebook demonstrates how to retrieve metadata associated to the paper [A SARS-CoV-2 cytopathicity dataset generated by high-content screening of a large drug repurposing collection](https://doi.org/10.1038/s41597-021-00848-4) and available in IDR at [idr0094-ellinger-sarscov2](https://idr.openmicroscopy.org/search/?query=Name:idr0094). Over 300 compounds were used in this investigation. This notebook allows the user to calculate the half maximal inhibitory concentration (IC50) for each compound. IC50 is a measure of the potency of a substance in inhibiting a specific biological or biochemical function. IC50 is a quantitative measure that indicates how much of a particular inhibitory substance (e.g. drug) is needed to inhibit, in vitro, a given biological process or biological component by 50%. User can download the IC50 for each compound used in that study The notebook can be launched in [My Binder](https://mybinder.org/v2/gh/IDR/idr0094-ellinger-sarscov2/master?urlpath=notebooks%2Fnotebooks%2Fidr0094-ic50.ipynb%3FscreenId%3D2603). A shiny app is also available for dynamic plotting of the IC50 curve for each compound. This R shiny app can be launched in [My Binder](https://mybinder.org/v2/gh/IDR/idr0094-ellinger-sarscov2/master?urlpath=shiny/apps/) # Inputs Parameters needed to configure the workflow: **screenId**: Identifier of a screen in IDR # Ouputs Output file generated: **ic50.csv**: Comma separate value file containing the IC50 for each compound