#!/usr/bin/env python3

import os
import sys
import errno
import argparse


def parse_args(args=None):
    Description = "Reformat nf-core/rnaseq samplesheet file and check its contents."
    Epilog = "Example usage: python check_samplesheet.py <FILE_IN> <FILE_OUT>"

    parser = argparse.ArgumentParser(description=Description, epilog=Epilog)
    parser.add_argument("FILE_IN", help="Input samplesheet file.")
    parser.add_argument("FILE_OUT", help="Output file.")
    return parser.parse_args(args)


def make_dir(path):
    if len(path) > 0:
        try:
            os.makedirs(path)
        except OSError as exception:
            if exception.errno != errno.EEXIST:
                raise exception


def print_error(error, context="Line", context_str=""):
    error_str = f"ERROR: Please check samplesheet -> {error}"
    if context != "" and context_str != "":
        error_str = f"ERROR: Please check samplesheet -> {error}\n{context.strip()}: '{context_str.strip()}'"
    print(error_str)
    sys.exit(1)


def check_samplesheet(file_in, file_out):
    """
    This function checks that the samplesheet follows the following structure:

    sample,fastq_1,fastq_2,strandedness
    SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz,forward
    SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz,forward
    SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz,,forward

    For an example see:
    https://github.com/nf-core/test-datasets/blob/rnaseq/samplesheet/v3.1/samplesheet_test.csv
    """

    sample_mapping_dict = {}
    with open(file_in, "r", encoding="utf-8-sig") as fin:

        ## Check header
        MIN_COLS = 3
        HEADER = ["sample", "fastq_1", "fastq_2", "strandedness"]
        header = [x.strip('"') for x in fin.readline().strip().split(",")]
        if header[: len(HEADER)] != HEADER:
            print(f"ERROR: Please check samplesheet header -> {','.join(header)} != {','.join(HEADER)}")
            sys.exit(1)

        ## Check sample entries
        for line in fin:
            if line.strip():
                lspl = [x.strip().strip('"') for x in line.strip().split(",")]

                ## Check valid number of columns per row
                if len(lspl) < len(HEADER):
                    print_error(
                        f"Invalid number of columns (minimum = {len(HEADER)})!",
                        "Line",
                        line,
                    )

                num_cols = len([x for x in lspl if x])
                if num_cols < MIN_COLS:
                    print_error(
                        f"Invalid number of populated columns (minimum = {MIN_COLS})!",
                        "Line",
                        line,
                    )

                ## Check sample name entries
                sample, fastq_1, fastq_2, strandedness = lspl[: len(HEADER)]
                if sample.find(" ") != -1:
                    print(f"WARNING: Spaces have been replaced by underscores for sample: {sample}")
                    sample = sample.replace(" ", "_")
                if not sample:
                    print_error("Sample entry has not been specified!", "Line", line)

                ## Check FastQ file extension
                for fastq in [fastq_1, fastq_2]:
                    if fastq:
                        if fastq.find(" ") != -1:
                            print_error("FastQ file contains spaces!", "Line", line)
                        if not fastq.endswith(".fastq.gz") and not fastq.endswith(".fq.gz"):
                            print_error(
                                "FastQ file does not have extension '.fastq.gz' or '.fq.gz'!",
                                "Line",
                                line,
                            )

                ## Check strandedness
                strandednesses = ["unstranded", "forward", "reverse"]
                if strandedness:
                    if strandedness not in strandednesses:
                        print_error(
                            f"Strandedness must be one of '{', '.join(strandednesses)}'!",
                            "Line",
                            line,
                        )
                else:
                    print_error(
                        f"Strandedness has not been specified! Must be one of {', '.join(strandednesses)}.",
                        "Line",
                        line,
                    )

                ## Auto-detect paired-end/single-end
                sample_info = []  ## [single_end, fastq_1, fastq_2, strandedness]
                if sample and fastq_1 and fastq_2:  ## Paired-end short reads
                    sample_info = ["0", fastq_1, fastq_2, strandedness]
                elif sample and fastq_1 and not fastq_2:  ## Single-end short reads
                    sample_info = ["1", fastq_1, fastq_2, strandedness]
                else:
                    print_error("Invalid combination of columns provided!", "Line", line)

                ## Create sample mapping dictionary = {sample: [[ single_end, fastq_1, fastq_2, strandedness ]]}
                if sample not in sample_mapping_dict:
                    sample_mapping_dict[sample] = [sample_info]
                else:
                    if sample_info in sample_mapping_dict[sample]:
                        print_error("Samplesheet contains duplicate rows!", "Line", line)
                    else:
                        sample_mapping_dict[sample].append(sample_info)

    ## Write validated samplesheet with appropriate columns
    if len(sample_mapping_dict) > 0:
        out_dir = os.path.dirname(file_out)
        make_dir(out_dir)
        with open(file_out, "w") as fout:
            fout.write(",".join(["sample", "single_end", "fastq_1", "fastq_2", "strandedness"]) + "\n")
            for sample in sorted(sample_mapping_dict.keys()):

                ## Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end
                if not all(x[0] == sample_mapping_dict[sample][0][0] for x in sample_mapping_dict[sample]):
                    print_error(
                        f"Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end!",
                        "Sample",
                        sample,
                    )

                ## Check that multiple runs of the same sample are of the same strandedness
                if not all(x[-1] == sample_mapping_dict[sample][0][-1] for x in sample_mapping_dict[sample]):
                    print_error(
                        f"Multiple runs of a sample must have the same strandedness!",
                        "Sample",
                        sample,
                    )

                for idx, val in enumerate(sample_mapping_dict[sample]):
                    fout.write(",".join([f"{sample}_T{idx+1}"] + val) + "\n")
    else:
        print_error(f"No entries to process!", "Samplesheet: {file_in}")


def main(args=None):
    args = parse_args(args)
    check_samplesheet(args.FILE_IN, args.FILE_OUT)


if __name__ == "__main__":
    sys.exit(main())
