Workflow 1: Preprocessing
1.0

Workflow Type: Galaxy

Metatranscriptomics analysis using microbiome RNA-seq data (short)

Associated Tutorial

This workflows is part of the tutorial Workflow 1: Preprocessing, available in the GTN

Features

Thanks to...

Tutorial Author(s): Pratik Jagtap, Subina Mehta, Ray Sajulga, Bérénice Batut, Emma Leith, Praveen Kumar, Saskia Hiltemann, Paul Zierep, Engy Nasr

Workflow Author(s): Bérénice Batut, Pratik Jagtap, Subina Mehta, Ray Sajulga, Emma Leith, Praveen Kumar, Saskia Hiltemann, Paul Zierep

gtn star logo followed by the word workflows

Inputs

ID Name Description Type
T1A_forward T1A_forward n/a
  • File
T1A_reverse T1A_reverse n/a
  • File

Steps

ID Name Description
2 Quality control Forward toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.73+galaxy0
3 Quality control Reverse toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.73+galaxy0
4 Cutadapt toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.0+galaxy1
5 MultiQC toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy1
6 rRNA/rDNA selection toolshed.g2.bx.psu.edu/repos/rnateam/sortmerna/bg_sortmerna/2.1b.6
7 FASTQ interlacer toolshed.g2.bx.psu.edu/repos/devteam/fastq_paired_end_interlacer/fastq_paired_end_interlacer/1.2.0.1+galaxy0

Outputs

ID Name Description Type
FastQC on input dataset(s): RawData FastQC on input dataset(s): RawData n/a
  • File
FastQC on input dataset(s): Webpage FastQC on input dataset(s): Webpage n/a
  • File
_anonymous_output_1 _anonymous_output_1 n/a
  • File
_anonymous_output_2 _anonymous_output_2 n/a
  • File
Cutadapt on input dataset(s): Read 1 Output Cutadapt on input dataset(s): Read 1 Output n/a
  • File
_anonymous_output_3 _anonymous_output_3 n/a
  • File
Cutadapt on input dataset(s): Read 2 Output Cutadapt on input dataset(s): Read 2 Output n/a
  • File
MultiQC on input dataset(s): Stats MultiQC on input dataset(s): Stats n/a
  • File
MultiQC on input dataset(s): Webpage MultiQC on input dataset(s): Webpage n/a
  • File
_anonymous_output_4 _anonymous_output_4 n/a
  • File
_anonymous_output_5 _anonymous_output_5 n/a
  • File
_anonymous_output_6 _anonymous_output_6 n/a
  • File
_anonymous_output_7 _anonymous_output_7 n/a
  • File
_anonymous_output_8 _anonymous_output_8 n/a
  • File
_anonymous_output_9 _anonymous_output_9 n/a
  • File
FASTQ interlacer singles from input dataset(s) FASTQ interlacer singles from input dataset(s) n/a
  • File
FASTQ interlacer pairs from input dataset(s) FASTQ interlacer pairs from input dataset(s) n/a
  • File

Version History

1.0 (earliest) Created 25th Jun 2024 at 10:56 by Helena Rasche

Added/updated 4 files


Open master 8897cc6
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Views: 325   Downloads: 78

Created: 25th Jun 2024 at 10:56

Last updated: 25th Jun 2024 at 10:56

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