Workflow Type: Galaxy
Open
Frozen
encyclopedia- DIA Metaproteomics
Associated Tutorial
This workflows is part of the tutorial EncyclopeDIA, available in the GTN
Features
- Includes Galaxy Workflow Tests
- Includes a Galaxy Workflow Report
Thanks to...
Workflow Author(s): GalaxyP
Tutorial Author(s): Emma Leith, Subina Mehta, James Johnson, Pratik Jagtap, Timothy J. Griffin
Inputs
ID | Name | Description | Type |
---|---|---|---|
BACKGROUND PROTEIN FASTA FILE | #main/BACKGROUND PROTEIN FASTA FILE | Input Protein Database file |
|
EXPERIMENTAL DIA RAW FILES (WIDE) | #main/EXPERIMENTAL DIA RAW FILES (WIDE) | Input raw files |
|
GPF DIA RAW Files (Dataset collection) | #main/GPF DIA RAW Files (Dataset collection) | encyclopedia- Gas phase fractionation files for library |
|
SPECTRAL OR PROSIT LIBRARY | #main/SPECTRAL OR PROSIT LIBRARY | Library from Prosit |
|
Steps
ID | Name | Description |
---|---|---|
4 | Converting RAW to mzml | convert to mzml for encyclopedia toolshed.g2.bx.psu.edu/repos/galaxyp/msconvert/msconvert/3.0.20287.2 |
5 | msconvert | toolshed.g2.bx.psu.edu/repos/galaxyp/msconvert/msconvert/3.0.20287.2 |
6 | Library searching | Searching the mzml files against the Prosit library and the protein FASTA database. toolshed.g2.bx.psu.edu/repos/galaxyp/encyclopedia_searchtolib/encyclopedia_searchtolib/1.2.2+galaxy0 |
7 | EncyclopeDIA Quantify | toolshed.g2.bx.psu.edu/repos/galaxyp/encyclopedia_quantify/encyclopedia_quantify/1.2.2+galaxy0 |
Outputs
ID | Name | Description | Type |
---|---|---|---|
CHROMATOGRAM LIBRARY | #main/CHROMATOGRAM LIBRARY | n/a |
|
Experimental-dataset-MZML | #main/Experimental-dataset-MZML | n/a |
|
GPF_MZML | #main/GPF_MZML | n/a |
|
LOG | #main/LOG | n/a |
|
PEPTIDE QUANTITATION OUTPUT | #main/PEPTIDE QUANTITATION OUTPUT | n/a |
|
PROTEIN QUANTITATION OUTPUT | #main/PROTEIN QUANTITATION OUTPUT | n/a |
|
QUANT LIBRARY | #main/QUANT LIBRARY | n/a |
|
Quant-LOG | #main/Quant-LOG | n/a |
|
Version History
2.0 (latest) Created 9th Sep 2024 at 14:23 by Helena Rasche
Added/updated 4 files
Open
master
e1af47d
4.0 (earliest) Created 25th Jun 2024 at 11:03 by Helena Rasche
Added/updated 4 files
Frozen
4.0
144d8a5
Creators and Submitter
Creators
Not specifiedSubmitter
Discussion Channel
Tool
Activity
Views: 740 Downloads: 245
Created: 25th Jun 2024 at 11:03
Last updated: 9th Sep 2024 at 14:23
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