Research Object Crate for Purge duplicates from hifiasm assembly v1.0 (HiFi genome assembly stage 3)
Version 1

Workflow Type: Galaxy

Optional workflow to purge duplicates from the contig assembly.

Infrastructure_deployment_metadata: Galaxy Australia (Galaxy)

Inputs

ID Name Description Type
HiFiASM 1o assembly HiFiASM 1o assembly Primary assembly input from hifiasm workflow, in FASTA format. n/a
HiFi reads as FASTQ HiFi reads as FASTQ Raw HiFi reads input, in FASTQ format. n/a

Steps

ID Name Description
0 HiFiASM 1o assembly Primary assembly input from hifiasm workflow, in FASTA format.
1 HiFi reads as FASTQ Raw HiFi reads input, in FASTQ format.
2 Step 1. Base-level coverage toolshed.g2.bx.psu.edu/repos/iuc/purge_dups/purge_dups/1.2.5+galaxy3
3 Step 1. Run minimap2 to align pacbio data and generate paf files, toolshed.g2.bx.psu.edu/repos/iuc/minimap2/minimap2/2.20+galaxy2
4 Step 1. Split an assembly and do a self-self alignment toolshed.g2.bx.psu.edu/repos/iuc/minimap2/minimap2/2.20+galaxy2
5 Step 1. then calculate read depth histogram and base-level read depth. toolshed.g2.bx.psu.edu/repos/iuc/purge_dups/purge_dups/1.2.5+galaxy3
6 Step 2. Purge haplotigs and overlaps toolshed.g2.bx.psu.edu/repos/iuc/purge_dups/purge_dups/1.2.5+galaxy3
7 Step 3. Get purged primary and haplotig sequences from draft assembly toolshed.g2.bx.psu.edu/repos/iuc/purge_dups/purge_dups/1.2.5+galaxy3
8 Haplotype fasta toolshed.g2.bx.psu.edu/repos/iuc/fasta_stats/fasta-stats/1.0.3

Outputs

ID Name Description Type
split_fasta split_fasta n/a
alignment_output alignment_output n/a
alignment_output alignment_output n/a
stat_file stat_file n/a
pbcstat_cov pbcstat_cov n/a
pbcstat_wig pbcstat_wig n/a
hist hist n/a
calcuts_log calcuts_log n/a
calcuts_tab calcuts_tab n/a
purge_dups_log purge_dups_log n/a
purge_dups_bed purge_dups_bed n/a
get_seqs_hap get_seqs_hap n/a
get_seqs_purged get_seqs_purged n/a
stats stats n/a

Version History

Version 1 (earliest) Created 23rd Nov 2021 at 10:26 by Stian Soiland-Reyes

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Frozen Version-1 ab0934a
help Creators and Submitter
Creators
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Additional credit

Gareth Price

Submitter
Activity

Views: 183

Created: 23rd Nov 2021 at 10:26

Last updated: 26th Jan 2022 at 12:07

Last used: 27th Sep 2022 at 10:07

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