yi.sun@embl.de

The images are two time points of a time lapse experiment where the INCENP gene was subjected to siRNA knock-down. The data are taken from the published mitocheck screen. In this screen the authors carried out a genome-wide phenotypic profiling of each of the ~21,000 human protein-coding genes by two-day live imaging of fluorescently labelled chromosomes.

Detecting a set of objects in an image, counting them and measuring certain characteristics about their morphology is probably the most frequently occurring task in bioimage analysis. Depending on the image, even this task could become quite challenging and the workflow could become quite complex. Here we start with a relatively simple image where combining a minimal set of image analysis components into a simple workflow does the job.