Workflows
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This workflow uses the decoupler tool in Galaxy to generate pseudobulk counts from an annotated AnnData file obtained from scRNA-seq analysis. Following the pseudobulk step, differential expression genes (DEG) are calculated using the edgeR tool. The workflow also includes data sanitation steps to ensure smooth operation of edgeR and minimizing potential issues. Additionally, a Volcano plot tool is used to visualize the results after the DEG analysis.
Associated Tutorial
This workflows is part ...
Associated Tutorial
This workflows is part of the tutorial Evaluating Reference Data for Bulk RNA Deconvolution, available in the GTN
Features
- Includes Galaxy Workflow Tests
- Includes a [Galaxy Workflow ...
Associated Tutorial
This workflows is part of the tutorial Evaluating Reference Data for Bulk RNA Deconvolution, available in the GTN
Features
- Includes Galaxy Workflow Tests
- Includes a [Galaxy Workflow ...
Associated Tutorial
This workflows is part of the tutorial Evaluating Reference Data for Bulk RNA Deconvolution, available in the GTN
Features
- Includes Galaxy Workflow Tests
- Includes a [Galaxy Workflow ...
Associated Tutorial
This workflows is part of the tutorial Evaluating Reference Data for Bulk RNA Deconvolution, available in the GTN
Features
- Includes Galaxy Workflow Tests
- Includes a [Galaxy Workflow ...
This is the workflow for the Clustering 3K PBMCs with Seurat tutorial if you are using the separate preprocessing tools (NormalizeData, FindVariableFeatures, ScaleData).
Associated Tutorial
This workflows is part of the tutorial Clustering 3K PBMCs with Seurat, available in the GTN
Features
- Includes [Galaxy Workflow ...
This is the workflow for the Clustering 3K PBMCs with Seurat tutorial if you use SCTransform for preprocessing.
Associated Tutorial
This workflows is part of the tutorial Clustering 3K PBMCs with Seurat, available in the GTN
Features
- Includes Galaxy Workflow Tests ...