Workflow Type: Galaxy
Open
Frozen
Analysis of variation within individual COVID-19 samples (Paired End Illumina). Does not use paured
Associated Tutorial
This workflows is part of the tutorial Simple COVID-19 - PE Variation, available in the GTN
Thanks to...
Tutorial Author(s): Marius van den Beek, Dave Clements, Daniel Blankenberg, Anton Nekrutenko
Tutorial Contributor(s): Wolfgang Maier
Inputs
ID | Name | Description | Type |
---|---|---|---|
NC_045512.2 fasta file | #main/NC_045512.2 fasta file | Contains SARS-CoV-2 reference sequence |
|
SRA Manifest | #main/SRA Manifest | n/a |
|
Steps
ID | Name | Description |
---|---|---|
2 | Faster Download and Extract Reads in FASTQ | toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/2.10.7+galaxy1 |
3 | fastp | toolshed.g2.bx.psu.edu/repos/iuc/fastp/fastp/0.20.1+galaxy0 |
4 | Map with BWA-MEM | toolshed.g2.bx.psu.edu/repos/devteam/bwa/bwa_mem/0.7.17.1 |
5 | MarkDuplicates | toolshed.g2.bx.psu.edu/repos/devteam/picard/picard_MarkDuplicates/2.18.2.2 |
6 | Realign reads | toolshed.g2.bx.psu.edu/repos/iuc/lofreq_viterbi/lofreq_viterbi/2.1.5+galaxy0 |
7 | Samtools stats | toolshed.g2.bx.psu.edu/repos/devteam/samtools_stats/samtools_stats/2.0.2+galaxy2 |
8 | Insert indel qualities | toolshed.g2.bx.psu.edu/repos/iuc/lofreq_indelqual/lofreq_indelqual/2.1.5+galaxy0 |
9 | Call variants | toolshed.g2.bx.psu.edu/repos/iuc/lofreq_call/lofreq_call/2.1.5+galaxy0 |
10 | SnpEff eff: | toolshed.g2.bx.psu.edu/repos/iuc/snpeff_sars_cov_2/snpeff_sars_cov_2/4.5covid19 |
11 | SnpSift Extract Fields | toolshed.g2.bx.psu.edu/repos/iuc/snpsift/snpSift_extractFields/4.3+t.galaxy0 |
12 | MultiQC | toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.8+galaxy0 |
Outputs
ID | Name | Description | Type |
---|---|---|---|
MultiQC Report PE | #main/MultiQC Report PE | n/a |
|
Realigned Alignments with Indel Qualities | #main/Realigned Alignments with Indel Qualities | n/a |
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SnpSift tabular output | #main/SnpSift tabular output | n/a |
|
_anonymous_output_3 | #main/_anonymous_output_3 | n/a |
|
_anonymous_output_4 | #main/_anonymous_output_4 | n/a |
|
Version History
1.0 (latest) Created 16th Jul 2024 at 14:09 by Helena Rasche
Added/updated 4 files
Open
master
03d0cad
7.0 (earliest) Created 25th Jun 2024 at 10:54 by Helena Rasche
Added/updated 4 files
Frozen
7.0
0820dab
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Views: 469 Downloads: 149
Created: 25th Jun 2024 at 10:54
Last updated: 25th Jun 2024 at 10:54
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