Workflow Type: Galaxy

Analysis of variation within individual COVID-19 samples (Paired End Illumina). Does not use paured

Associated Tutorial

This workflows is part of the tutorial Simple COVID-19 - PE Variation, available in the GTN

Thanks to...

Tutorial Author(s): Marius van den Beek, Dave Clements, Daniel Blankenberg, Anton Nekrutenko

Tutorial Contributor(s): Wolfgang Maier

gtn star logo followed by the word workflows

Inputs

ID Name Description Type
NC_045512.2 fasta file #main/NC_045512.2 fasta file Contains SARS-CoV-2 reference sequence
  • File
SRA Manifest #main/SRA Manifest n/a
  • File

Steps

ID Name Description
2 Faster Download and Extract Reads in FASTQ toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/2.10.7+galaxy1
3 fastp toolshed.g2.bx.psu.edu/repos/iuc/fastp/fastp/0.20.1+galaxy0
4 Map with BWA-MEM toolshed.g2.bx.psu.edu/repos/devteam/bwa/bwa_mem/0.7.17.1
5 MarkDuplicates toolshed.g2.bx.psu.edu/repos/devteam/picard/picard_MarkDuplicates/2.18.2.2
6 Realign reads toolshed.g2.bx.psu.edu/repos/iuc/lofreq_viterbi/lofreq_viterbi/2.1.5+galaxy0
7 Samtools stats toolshed.g2.bx.psu.edu/repos/devteam/samtools_stats/samtools_stats/2.0.2+galaxy2
8 Insert indel qualities toolshed.g2.bx.psu.edu/repos/iuc/lofreq_indelqual/lofreq_indelqual/2.1.5+galaxy0
9 Call variants toolshed.g2.bx.psu.edu/repos/iuc/lofreq_call/lofreq_call/2.1.5+galaxy0
10 SnpEff eff: toolshed.g2.bx.psu.edu/repos/iuc/snpeff_sars_cov_2/snpeff_sars_cov_2/4.5covid19
11 SnpSift Extract Fields toolshed.g2.bx.psu.edu/repos/iuc/snpsift/snpSift_extractFields/4.3+t.galaxy0
12 MultiQC toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.8+galaxy0

Outputs

ID Name Description Type
MultiQC Report PE #main/MultiQC Report PE n/a
  • File
Realigned Alignments with Indel Qualities #main/Realigned Alignments with Indel Qualities n/a
  • File
SnpSift tabular output #main/SnpSift tabular output n/a
  • File
_anonymous_output_3 #main/_anonymous_output_3 n/a
  • File
_anonymous_output_4 #main/_anonymous_output_4 n/a
  • File

Version History

1.0 (latest) Created 16th Jul 2024 at 14:09 by Helena Rasche

Added/updated 4 files


Open master 03d0cad

7.0 (earliest) Created 25th Jun 2024 at 10:54 by Helena Rasche

Added/updated 4 files


Frozen 7.0 0820dab
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Views: 192   Downloads: 77

Created: 25th Jun 2024 at 10:54

Last updated: 25th Jun 2024 at 10:54

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