Workflow Type: Galaxy
Open
This workflow is created as part of a tutorial listed on GTN. The workflow shows the steps in human copy number variance detection using the Contrl_FREEC tool.
Associated Tutorial
This workflows is part of the tutorial Somatic-Variant-Discovery-from-WES-Data-Using-Control-FREEC, available in the GTN
Features
- Includes Galaxy Workflow Tests
Thanks to...
Tutorial Author(s): Khaled Jum'ah, Katarzyna Kamieniecka, Wolfgang Maier, David Salgado, Krzysztof Poterlowicz
Workflow Author(s): khaled Jumah, Katarzyna Kamieniecka, Wolfgang Maier, Krzysztof Poterlowicz, poterlowicz-lab
Inputs
ID | Name | Description | Type |
---|---|---|---|
Normal_r1.fastq.gz | Normal_r1.fastq.gz | n/a |
|
Normal_r2.fastq.gz | Normal_r2.fastq.gz | n/a |
|
Tumor_r1.fastq.gz | Tumor_r1.fastq.gz | n/a |
|
Tumor_r2.fastq.gz | Tumor_r2.fastq.gz | n/a |
|
capture_targets_chr5_12_17.bed | capture_targets_chr5_12_17.bed | n/a |
|
Steps
ID | Name | Description |
---|---|---|
3 | Create text file | toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_text_file_with_recurring_lines/1.1.0 |
6 | FastQC | toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.74+galaxy0 |
7 | Trimmomatic | toolshed.g2.bx.psu.edu/repos/pjbriggs/trimmomatic/trimmomatic/0.39+galaxy0 |
8 | FastQC | toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.74+galaxy0 |
9 | FastQC | toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.74+galaxy0 |
10 | Trimmomatic | toolshed.g2.bx.psu.edu/repos/pjbriggs/trimmomatic/trimmomatic/0.39+galaxy0 |
11 | FastQC | toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.74+galaxy0 |
12 | FastQC | toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.74+galaxy0 |
13 | Map with BWA-MEM | toolshed.g2.bx.psu.edu/repos/devteam/bwa/bwa_mem/0.7.17.2 |
14 | FastQC | toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.74+galaxy0 |
15 | FastQC | toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.74+galaxy0 |
16 | Map with BWA-MEM | toolshed.g2.bx.psu.edu/repos/devteam/bwa/bwa_mem/0.7.17.2 |
17 | FastQC | toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.74+galaxy0 |
18 | MultiQC | toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy1 |
19 | Build list | __BUILD_LIST__ |
20 | MultiQC | toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy1 |
21 | Relabel identifiers | __RELABEL_FROM_FILE__ |
22 | Samtools view | toolshed.g2.bx.psu.edu/repos/iuc/samtools_view/samtools_view/1.15.1+galaxy0 |
23 | RmDup | toolshed.g2.bx.psu.edu/repos/devteam/samtools_rmdup/samtools_rmdup/2.0.1 |
24 | BamLeftAlign | toolshed.g2.bx.psu.edu/repos/devteam/freebayes/bamleftalign/1.3.6 |
25 | Samtools calmd | toolshed.g2.bx.psu.edu/repos/devteam/samtools_calmd/samtools_calmd/2.0.3 |
26 | Samtools view | toolshed.g2.bx.psu.edu/repos/iuc/samtools_view/samtools_view/1.15.1+galaxy0 |
27 | Samtools view | toolshed.g2.bx.psu.edu/repos/iuc/samtools_view/samtools_view/1.15.1+galaxy0 |
28 | Select | Grep1 |
29 | Replace Text | toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/1.1.2 |
30 | Samtools view | toolshed.g2.bx.psu.edu/repos/iuc/samtools_view/samtools_view/1.15.1+galaxy0 |
31 | Extract Tumor read | __EXTRACT_DATASET__ |
32 | Extract normal read | __EXTRACT_DATASET__ |
33 | Control-FREEC | toolshed.g2.bx.psu.edu/repos/iuc/control_freec/control_freec/11.6+galaxy2 |
34 | Circos | toolshed.g2.bx.psu.edu/repos/iuc/circos/circos/0.69.8+galaxy9 |
Outputs
ID | Name | Description | Type |
---|---|---|---|
out_sample_info | out_sample_info | n/a |
|
out_sample_coord | out_sample_coord | n/a |
|
out_control_raw | out_control_raw | n/a |
|
out_sample_raw | out_sample_raw | n/a |
|
out_ratio_log2_circos | out_ratio_log2_circos | n/a |
|
out_chr_sorted_circos | out_chr_sorted_circos | n/a |
|
out_gc_profile | out_gc_profile | n/a |
|
out_mg_log2_png | out_mg_log2_png | n/a |
|
out_mg_png | out_mg_png | n/a |
|
out_sample_subclones | out_sample_subclones | n/a |
|
out_sample_ratio | out_sample_ratio | n/a |
|
output_png | output_png | n/a |
|
Version History
1.0 (earliest) Created 25th Jun 2024 at 10:59 by Helena Rasche
Added/updated 4 files
Open
master
24fe8ae
Creators and Submitter
Creators
Not specifiedSubmitter
Discussion Channel
Activity
Views: 297 Downloads: 83
Created: 25th Jun 2024 at 10:59
Last updated: 25th Jun 2024 at 10:59
Tags
Attributions
None