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Design plasmids encoding predicted pathways by using the BASIC assembly method.
Associated Tutorial
This workflows is part of the tutorial Genetic Design (BASIC Assembly), available in the GTN
Thanks to...
Tutorial Author(s): Kenza Bazi-Kabbaj, Thomas Duigou, Joan Hérisson, Guillaume Gricourt, Ioana Popescu, Jean-Loup Faulon
Inputs
| ID | Name | Description | Type |
|---|---|---|---|
| DNA-Bot settings | #main/DNA-Bot settings | n/a |
|
| Enzyme taxon IDs | #main/Enzyme taxon IDs | Comma separated values |
|
| Host taxon ID | #main/Host taxon ID | n/a |
|
| Linkers and user parts | #main/Linkers and user parts | n/a |
|
| Pathway (SBML) | #main/Pathway (SBML) | n/a |
|
Steps
| ID | Name | Description |
|---|---|---|
| 5 | Selenzyme | Performs enzyme selection from a reaction query. toolshed.g2.bx.psu.edu/repos/tduigou/selenzy/selenzy-wrapper/0.2.0 |
| 6 | BasicDesign | Extracts enzyme IDs from rpSBML files. toolshed.g2.bx.psu.edu/repos/tduigou/rpbasicdesign/rpbasicdesign/0.3.4 |
| 7 | DNA-Bot | DNA assembly using BASIC on OpenTrons. toolshed.g2.bx.psu.edu/repos/tduigou/dnabot/dnabot/3.1.0 |
Outputs
| ID | Name | Description | Type |
|---|---|---|---|
| Uniprot IDs | #main/Uniprot IDs | n/a |
|
| _anonymous_output_1 | #main/_anonymous_output_1 | n/a |
|
| _anonymous_output_2 | #main/_anonymous_output_2 | n/a |
|
| _anonymous_output_3 | #main/_anonymous_output_3 | n/a |
|
| _anonymous_output_4 | #main/_anonymous_output_4 | n/a |
|
| _anonymous_output_5 | #main/_anonymous_output_5 | n/a |
|
| _anonymous_output_6 | #main/_anonymous_output_6 | n/a |
|
Version History
1.0 (latest) Created 16th Jul 2024 at 14:06 by Helena Rasche
Added/updated 4 files
Open
master
b26f00e
3.0 (earliest) Created 25th Jun 2024 at 11:03 by Helena Rasche
Added/updated 4 files
Frozen
3.0
549296f
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Views: 1386 Downloads: 548 Runs: 0
Created: 25th Jun 2024 at 11:03
Last updated: 25th Jun 2024 at 11:03
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