Workflow Type: Galaxy

roscoff hackathon

Associated Tutorial

This workflows is part of the tutorial trinity NG, available in the GTN

Thanks to...

Tutorial Author(s): Anthony Bretaudeau, Gildas Le Corguillé, Erwan Corre, Xi Liu

gtn star logo followed by the word workflows

Inputs

ID Name Description Type
Collection of R1 reads Collection of R1 reads n/a
  • File[]
Collection of R2 reads Collection of R2 reads n/a
  • File[]
Samples description Samples description The list of samples and replicates. Can be created with the 'Describe samples and replicates' tool
  • File

Steps

ID Name Description
20 Trinotate toolshed.g2.bx.psu.edu/repos/iuc/trinotate/trinotate/3.1.1
21 Differential expression analysis toolshed.g2.bx.psu.edu/repos/iuc/trinity_run_de_analysis/trinity_run_de_analysis/2.8.4
22 Extract and cluster differentially expressed transcripts toolshed.g2.bx.psu.edu/repos/iuc/trinity_analyze_diff_expr/trinity_analyze_diff_expr/2.8.4
23 Partition genes into expression clusters toolshed.g2.bx.psu.edu/repos/iuc/trinity_define_clusters_by_cutting_tree/trinity_define_clusters_by_cutting_tree/2.8.4
2 Describe samples toolshed.g2.bx.psu.edu/repos/iuc/describe_samples/describe_samples/2.8.4
5 FastQC toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.69
4 FastQC toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.69
7 MultiQC toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.2.0
6 Trimmomatic toolshed.g2.bx.psu.edu/repos/pjbriggs/trimmomatic/trimmomatic/0.32.3
9 FastQC toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.69
8 FastQC toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.69
11 MultiQC toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.2.0
10 Trinity toolshed.g2.bx.psu.edu/repos/iuc/trinity/trinity/2.8.4
13 Build expression matrix toolshed.g2.bx.psu.edu/repos/iuc/trinity_abundance_estimates_to_matrix/trinity_abundance_estimates_to_matrix/2.8.4
12 Align reads and estimate abundance toolshed.g2.bx.psu.edu/repos/iuc/trinity_align_and_estimate_abundance/trinity_align_and_estimate_abundance/2.8.4
15 Filter low expression transcripts toolshed.g2.bx.psu.edu/repos/iuc/trinity_filter_low_expr_transcripts/trinity_filter_low_expr_transcripts/2.8.4
14 RNASeq samples quality check toolshed.g2.bx.psu.edu/repos/iuc/trinity_samples_qccheck/trinity_samples_qccheck/2.8.4
17 Align reads and estimate abundance toolshed.g2.bx.psu.edu/repos/iuc/trinity_align_and_estimate_abundance/trinity_align_and_estimate_abundance/2.8.4
16 Compute contig Ex90N50 statistic and Ex90 transcript count toolshed.g2.bx.psu.edu/repos/iuc/trinity_contig_exn50_statistic/trinity_contig_exn50_statistic/2.8.4
19 Build expression matrix toolshed.g2.bx.psu.edu/repos/iuc/trinity_abundance_estimates_to_matrix/trinity_abundance_estimates_to_matrix/2.8.4
18 TransDecoder toolshed.g2.bx.psu.edu/repos/iuc/transdecoder/transdecoder/3.0.1

Outputs

ID Name Description Type
_anonymous_output_1 _anonymous_output_1 n/a
  • File
_anonymous_output_2 _anonymous_output_2 n/a
  • File
_anonymous_output_3 _anonymous_output_3 n/a
  • File
_anonymous_output_4 _anonymous_output_4 n/a
  • File
_anonymous_output_5 _anonymous_output_5 n/a
  • File
_anonymous_output_6 _anonymous_output_6 n/a
  • File
_anonymous_output_7 _anonymous_output_7 n/a
  • File
_anonymous_output_11 _anonymous_output_11 n/a
  • File
_anonymous_output_12 _anonymous_output_12 n/a
  • File
_anonymous_output_13 _anonymous_output_13 n/a
  • File
_anonymous_output_14 _anonymous_output_14 n/a
  • File
_anonymous_output_15 _anonymous_output_15 n/a
  • File
_anonymous_output_16 _anonymous_output_16 n/a
  • File

Version History

1.0 (latest) Created 16th Jul 2024 at 14:05 by Helena Rasche

Added/updated 4 files


Open master caa24a6

2.0 (earliest) Created 25th Jun 2024 at 11:22 by Helena Rasche

Added/updated 4 files


Frozen 2.0 7c61af1
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Views: 408   Downloads: 144

Created: 25th Jun 2024 at 11:22

Last updated: 25th Jun 2024 at 11:22

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