mRNA-Seq BY-COVID Pipeline: Counts
1.0

Workflow Type: Galaxy

This portion of the workflow produces sets of feature Counts ready for analysis by limma/etc.

Associated Tutorial

This workflows is part of the tutorial mRNA-Seq BY-COVID Pipeline: Counts, available in the GTN

Features

Thanks to...

Tutorial Author(s): Marek Ostaszewski, Matti Hoch, Iacopo Cristoferi, Myrthe van Baardwijk, Saskia Hiltemann, Helena Rasche

Tutorial Contributor(s): Helena Rasche, Matti Hoch, Mira Kuntz, José Manuel Domínguez, Sanjay Kumar Srikakulam, Björn Grüning

Workflow Author(s): Iacopo Cristoferi, Helena Rasche, Clinical Bioinformatics Unit, Pathology Department, Eramus Medical Center

Funder(s): BeYond-COVID

gtn star logo followed by the word workflows

Inputs

ID Name Description Type
UCSC Genome #main/UCSC Genome Export of UCSC Genome, just the genes.
  • File
mRNA-Seq Reads #main/mRNA-Seq Reads Input list of fastqsanger format sequencing data
  • array containing
    • File

Steps

ID Name Description
2 FastQC toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.74+galaxy0
3 Cutadapt toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.4+galaxy0
4 FastQC toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.74+galaxy0
5 HISAT2 toolshed.g2.bx.psu.edu/repos/iuc/hisat2/hisat2/2.2.1+galaxy1
6 featureCounts toolshed.g2.bx.psu.edu/repos/iuc/featurecounts/featurecounts/2.0.3+galaxy1
7 Read Distribution toolshed.g2.bx.psu.edu/repos/nilesh/rseqc/rseqc_read_distribution/5.0.1+galaxy2
8 Gene Body Coverage (BAM) toolshed.g2.bx.psu.edu/repos/nilesh/rseqc/rseqc_geneBody_coverage/5.0.1+galaxy2
9 Multi QC raw reads toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy1

Outputs

ID Name Description Type
html_report #main/html_report n/a
  • File
output_feature_lengths #main/output_feature_lengths n/a
  • File
output_short #main/output_short n/a
  • File

Version History

1.0 (earliest) Created 25th Jun 2024 at 11:25 by Helena Rasche

Added/updated 4 files


Open master 8530f83
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Views: 470   Downloads: 110

Created: 25th Jun 2024 at 11:25

Last updated: 25th Jun 2024 at 11:25

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