Workflow Type: Galaxy
Open
This portion of the workflow produces sets of feature Counts ready for analysis by limma/etc.
Associated Tutorial
This workflows is part of the tutorial mRNA-Seq BY-COVID Pipeline: Counts, available in the GTN
Features
- Includes Galaxy Workflow Tests
Thanks to...
Tutorial Author(s): Marek Ostaszewski, Matti Hoch, Iacopo Cristoferi, Myrthe van Baardwijk, Saskia Hiltemann, Helena Rasche
Tutorial Contributor(s): Helena Rasche, Matti Hoch, Mira Kuntz, José Manuel Domínguez, Sanjay Kumar Srikakulam, Björn Grüning
Workflow Author(s): Iacopo Cristoferi, Helena Rasche, Clinical Bioinformatics Unit, Pathology Department, Eramus Medical Center
Funder(s): BeYond-COVID
Inputs
ID | Name | Description | Type |
---|---|---|---|
UCSC Genome | #main/UCSC Genome | Export of UCSC Genome, just the genes. |
|
mRNA-Seq Reads | #main/mRNA-Seq Reads | Input list of fastqsanger format sequencing data |
|
Steps
ID | Name | Description |
---|---|---|
2 | FastQC | toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.74+galaxy0 |
3 | Cutadapt | toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.4+galaxy0 |
4 | FastQC | toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.74+galaxy0 |
5 | HISAT2 | toolshed.g2.bx.psu.edu/repos/iuc/hisat2/hisat2/2.2.1+galaxy1 |
6 | featureCounts | toolshed.g2.bx.psu.edu/repos/iuc/featurecounts/featurecounts/2.0.3+galaxy1 |
7 | Read Distribution | toolshed.g2.bx.psu.edu/repos/nilesh/rseqc/rseqc_read_distribution/5.0.1+galaxy2 |
8 | Gene Body Coverage (BAM) | toolshed.g2.bx.psu.edu/repos/nilesh/rseqc/rseqc_geneBody_coverage/5.0.1+galaxy2 |
9 | Multi QC raw reads | toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy1 |
Outputs
ID | Name | Description | Type |
---|---|---|---|
html_report | #main/html_report | n/a |
|
output_feature_lengths | #main/output_feature_lengths | n/a |
|
output_short | #main/output_short | n/a |
|
Version History
1.0 (earliest) Created 25th Jun 2024 at 11:25 by Helena Rasche
Added/updated 4 files
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Views: 344 Downloads: 75
Created: 25th Jun 2024 at 11:25
Last updated: 25th Jun 2024 at 11:25
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