TranscriptsAnnotation-wf
Version 1

Workflow Type: Common Workflow Language

Transcripts annotation workflow

Inputs

ID Name Description Type
transcriptsFile n/a n/a
  • File
singleBestOnly n/a n/a
  • boolean?
replace n/a n/a
  • ../utils/esl-reformat-replace.yaml#replace?
phmmerSeqdb n/a n/a
  • File
diamondSeqdb n/a n/a
  • File
i5Databases n/a n/a
  • Directory
i5Applications n/a n/a
  • ../tools/InterProScan/InterProScan-apps.yaml#apps[]?
i5OutputFormat n/a n/a
  • ../tools/InterProScan/InterProScan-protein_formats.yaml#protein_formats[]?
blockSize n/a n/a
  • float?
covariance_models n/a n/a
  • File[]
clanInfoFile n/a n/a
  • File
cmsearchCores n/a n/a
  • int
buscoMode n/a n/a
  • ../tools/BUSCO/BUSCO-assessment_modes.yaml#assessment_modes
buscoOutputName n/a n/a
  • string
buscoLineage n/a n/a
  • Directory

Steps

ID Name Description
identify_coding_regions Identifies candidate coding regions within transcript sequences n/a
remove_asterisks_and_reformat Removes asterisks characters from given peptide sequences n/a
functional_analysis n/a Matches are generated against predicted CDS, using a sub set of databases from InterPro.
calculate_phmmer_matches Calculates phmmer matches n/a
calculate_diamond_matches Calculates Diamond matches n/a
identify_nc_rna Identifies non-coding RNAs using Rfams covariance models n/a
run_transcriptome_assessment Performs transcriptome assessment using BUSCO n/a

Outputs

ID Name Description Type
peptide_sequences n/a n/a
  • File
coding_regions n/a n/a
  • File
gff3_output n/a n/a
  • File
bed_output n/a n/a
  • File
reformatted_sequences n/a n/a
  • File
i5Annotations n/a n/a
  • File
phmmer_matches n/a n/a
  • File
diamond_matches n/a n/a
  • File
deoverlapped_matches n/a n/a
  • File
busco_short_summary n/a n/a
  • File
busco_full_table n/a n/a
  • File
busco_missing_buscos n/a n/a
  • File
busco_hmmer_output n/a n/a
  • Directory
busco_translated_proteins n/a n/a
  • Directory
busco_blast_output n/a n/a
  • Directory

Version History

Version 1 (earliest) Created 25th Feb 2020 at 13:19 by Finn Bacall

Added/updated 25 files


Open master aa79813
help Creators and Submitter
Creators
Not specified
Submitter
Activity

Views: 1174   Downloads: 78

Created: 25th Feb 2020 at 13:19

Last updated: 25th Feb 2020 at 16:06

help Tags

This item has not yet been tagged.

help Attributions

None

Total size: 106 KB

Brought to you by:

Powered by
(v.1.14.0-pre)
Copyright © 2008 - 2023 The University of Manchester and HITS gGmbH