Workflow Type: Common Workflow Language
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Transcripts annotation workflow
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Inputs
| ID | Name | Description | Type |
|---|---|---|---|
| transcriptsFile | n/a | n/a |
|
| singleBestOnly | n/a | n/a |
|
| replace | n/a | n/a |
|
| phmmerSeqdb | n/a | n/a |
|
| diamondSeqdb | n/a | n/a |
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| i5Databases | n/a | n/a |
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| i5Applications | n/a | n/a |
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| i5OutputFormat | n/a | n/a |
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| blockSize | n/a | n/a |
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| covariance_models | n/a | n/a |
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| clanInfoFile | n/a | n/a |
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| cmsearchCores | n/a | n/a |
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| buscoMode | n/a | n/a |
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| buscoOutputName | n/a | n/a |
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| buscoLineage | n/a | n/a |
|
Steps
| ID | Name | Description |
|---|---|---|
| identify_coding_regions | Identifies candidate coding regions within transcript sequences | n/a |
| remove_asterisks_and_reformat | Removes asterisks characters from given peptide sequences | n/a |
| functional_analysis | n/a | Matches are generated against predicted CDS, using a sub set of databases from InterPro. |
| calculate_phmmer_matches | Calculates phmmer matches | n/a |
| calculate_diamond_matches | Calculates Diamond matches | n/a |
| identify_nc_rna | Identifies non-coding RNAs using Rfams covariance models | n/a |
| run_transcriptome_assessment | Performs transcriptome assessment using BUSCO | n/a |
Outputs
| ID | Name | Description | Type |
|---|---|---|---|
| peptide_sequences | n/a | n/a |
|
| coding_regions | n/a | n/a |
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| gff3_output | n/a | n/a |
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| bed_output | n/a | n/a |
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| reformatted_sequences | n/a | n/a |
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| i5Annotations | n/a | n/a |
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| phmmer_matches | n/a | n/a |
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| diamond_matches | n/a | n/a |
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| deoverlapped_matches | n/a | n/a |
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| busco_short_summary | n/a | n/a |
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| busco_full_table | n/a | n/a |
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| busco_missing_buscos | n/a | n/a |
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| busco_hmmer_output | n/a | n/a |
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| busco_translated_proteins | n/a | n/a |
|
| busco_blast_output | n/a | n/a |
|
Version History
Version 1 (earliest) Created 25th Feb 2020 at 13:19 by Finn Bacall
Added/updated 25 files
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Views: 2475 Downloads: 564
Created: 25th Feb 2020 at 13:19
Last updated: 25th Feb 2020 at 16:06
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