WorkflowsWhat is a Workflow?
This workflow take as input a collection of paired fastq. It will remove bad quality and adapters with cutadapt. Map with Bowtie2 end-to-end. Will remove reads on MT and unconcordant pairs and pairs with mapping quality below 30 and PCR duplicates. Will compute the pile-up on 5' +- 100bp. Will call peaks and count the number of reads falling in the 1kb region centered on the summit. Will plot the number of reads for each fragment length.
K-means clustering is a method of cluster analysis that aims to partition ''n'' points into ''k'' clusters in which each point belongs to the cluster with the nearest mean. It follows an iterative refinement strategy to find the centers of natural clusters in the data.
Submitter: Raül Sirvent