Workflow Type: Galaxy
Open
Galaxy workflow
Inputs
| ID | Name | Description | Type |
|---|---|---|---|
| Mus_musculus.GRCm38.100.gtf.gz | #main/Mus_musculus.GRCm38.100.gtf.gz | You can find the file containing the gene information for your species here: http://www.ensembl.org/info/data/ftp/index.html/ |
|
| Mus_musculus.GRCm38.cdna.all.fa | #main/Mus_musculus.GRCm38.cdna.all.fa | You can find this file for your species here: http://www.ensembl.org/info/data/ftp/index.html/ |
|
| Read 1 - containing cell barcode & UMI | #main/Read 1 - containing cell barcode & UMI | This should be a fast file containing the shorter read - i.e. the cell barcode and the unique molecular identifier |
|
| Read 2 - containing transcript | #main/Read 2 - containing transcript | This should be the read containing the transcript |
|
Steps
| ID | Name | Description |
|---|---|---|
| 4 | Transcript-gene mapping | toolshed.g2.bx.psu.edu/repos/ebi-gxa/gtf2gene_list/_ensembl_gtf2gene_list/1.52.0+galaxy0 |
| 5 | Gene information | toolshed.g2.bx.psu.edu/repos/ebi-gxa/gtf2gene_list/_ensembl_gtf2gene_list/1.52.0+galaxy0 |
| 6 | Alevin Processed | toolshed.g2.bx.psu.edu/repos/bgruening/alevin/alevin/1.9.0+galaxy2 |
| 7 | Alevin - no processing | toolshed.g2.bx.psu.edu/repos/bgruening/alevin/alevin/1.9.0+galaxy2 |
| 8 | Droplet rank plot - Alevin barcodes | toolshed.g2.bx.psu.edu/repos/ebi-gxa/droplet_barcode_plot/_dropletBarcodePlot/1.6.1+galaxy2 |
| 9 | Droplet rank plot - raw barcodes | toolshed.g2.bx.psu.edu/repos/ebi-gxa/droplet_barcode_plot/_dropletBarcodePlot/1.6.1+galaxy2 |
| 10 | salmonKallistoMtxTo10x | toolshed.g2.bx.psu.edu/repos/ebi-gxa/salmon_kallisto_mtx_to_10x/_salmon_kallisto_mtx_to_10x/0.0.1+galaxy6 |
| 11 | Join two Datasets | join1 |
| 12 | Annotated Gene Table | Cut1 |
| 13 | DropletUtils Read10x | toolshed.g2.bx.psu.edu/repos/ebi-gxa/dropletutils_read_10x/dropletutils_read_10x/1.0.4+galaxy0 |
| 14 | DropletUtils emptyDrops | toolshed.g2.bx.psu.edu/repos/ebi-gxa/dropletutils_empty_drops/dropletutils_empty_drops/1.0.4+galaxy0 |
| 15 | N701-400k-AnnData | toolshed.g2.bx.psu.edu/repos/ebi-gxa/sceasy_convert/sceasy_convert/0.0.5+galaxy1 |
Outputs
| ID | Name | Description | Type |
|---|---|---|---|
| Emptied-Object | #main/Emptied-Object | n/a |
|
| Emptied-Tabular Output | #main/Emptied-Tabular Output | n/a |
|
| N701-400k-AnnData | #main/N701-400k-AnnData | n/a |
|
| _anonymous_output_1 | #main/_anonymous_output_1 | n/a |
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| _anonymous_output_10 | #main/_anonymous_output_10 | n/a |
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| _anonymous_output_11 | #main/_anonymous_output_11 | n/a |
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| _anonymous_output_12 | #main/_anonymous_output_12 | n/a |
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| _anonymous_output_13 | #main/_anonymous_output_13 | n/a |
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| _anonymous_output_14 | #main/_anonymous_output_14 | n/a |
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| _anonymous_output_15 | #main/_anonymous_output_15 | n/a |
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| _anonymous_output_16 | #main/_anonymous_output_16 | n/a |
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| _anonymous_output_17 | #main/_anonymous_output_17 | n/a |
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| _anonymous_output_18 | #main/_anonymous_output_18 | n/a |
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| _anonymous_output_19 | #main/_anonymous_output_19 | n/a |
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| _anonymous_output_2 | #main/_anonymous_output_2 | n/a |
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| _anonymous_output_3 | #main/_anonymous_output_3 | n/a |
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| _anonymous_output_4 | #main/_anonymous_output_4 | n/a |
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| _anonymous_output_5 | #main/_anonymous_output_5 | n/a |
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| _anonymous_output_6 | #main/_anonymous_output_6 | n/a |
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| _anonymous_output_7 | #main/_anonymous_output_7 | n/a |
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| _anonymous_output_8 | #main/_anonymous_output_8 | n/a |
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| _anonymous_output_9 | #main/_anonymous_output_9 | n/a |
|
Version History
1 (earliest) Created 22nd Apr 2024 at 11:21 by Helena Rasche
Added/updated 3 files
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Views: 1087 Downloads: 283 Runs: 0
Created: 22nd Apr 2024 at 11:21
Last updated: 22nd Apr 2024 at 11:21
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https://orcid.org/0000-0001-9760-8992