Generating a single cell matrix using Alevin 1.9
1

Workflow Type: Galaxy

Galaxy workflow

Inputs

ID Name Description Type
Mus_musculus.GRCm38.100.gtf.gz #main/Mus_musculus.GRCm38.100.gtf.gz You can find the file containing the gene information for your species here: http://www.ensembl.org/info/data/ftp/index.html/
  • File
Mus_musculus.GRCm38.cdna.all.fa #main/Mus_musculus.GRCm38.cdna.all.fa You can find this file for your species here: http://www.ensembl.org/info/data/ftp/index.html/
  • File
Read 1 - containing cell barcode & UMI #main/Read 1 - containing cell barcode & UMI This should be a fast file containing the shorter read - i.e. the cell barcode and the unique molecular identifier
  • File
Read 2 - containing transcript #main/Read 2 - containing transcript This should be the read containing the transcript
  • File

Steps

ID Name Description
4 Transcript-gene mapping toolshed.g2.bx.psu.edu/repos/ebi-gxa/gtf2gene_list/_ensembl_gtf2gene_list/1.52.0+galaxy0
5 Gene information toolshed.g2.bx.psu.edu/repos/ebi-gxa/gtf2gene_list/_ensembl_gtf2gene_list/1.52.0+galaxy0
6 Alevin Processed toolshed.g2.bx.psu.edu/repos/bgruening/alevin/alevin/1.9.0+galaxy2
7 Alevin - no processing toolshed.g2.bx.psu.edu/repos/bgruening/alevin/alevin/1.9.0+galaxy2
8 Droplet rank plot - Alevin barcodes toolshed.g2.bx.psu.edu/repos/ebi-gxa/droplet_barcode_plot/_dropletBarcodePlot/1.6.1+galaxy2
9 Droplet rank plot - raw barcodes toolshed.g2.bx.psu.edu/repos/ebi-gxa/droplet_barcode_plot/_dropletBarcodePlot/1.6.1+galaxy2
10 salmonKallistoMtxTo10x toolshed.g2.bx.psu.edu/repos/ebi-gxa/salmon_kallisto_mtx_to_10x/_salmon_kallisto_mtx_to_10x/0.0.1+galaxy6
11 Join two Datasets join1
12 Annotated Gene Table Cut1
13 DropletUtils Read10x toolshed.g2.bx.psu.edu/repos/ebi-gxa/dropletutils_read_10x/dropletutils_read_10x/1.0.4+galaxy0
14 DropletUtils emptyDrops toolshed.g2.bx.psu.edu/repos/ebi-gxa/dropletutils_empty_drops/dropletutils_empty_drops/1.0.4+galaxy0
15 N701-400k-AnnData toolshed.g2.bx.psu.edu/repos/ebi-gxa/sceasy_convert/sceasy_convert/0.0.5+galaxy1

Outputs

ID Name Description Type
Emptied-Object #main/Emptied-Object n/a
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Emptied-Tabular Output #main/Emptied-Tabular Output n/a
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N701-400k-AnnData #main/N701-400k-AnnData n/a
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_anonymous_output_1 #main/_anonymous_output_1 n/a
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_anonymous_output_10 #main/_anonymous_output_10 n/a
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_anonymous_output_11 #main/_anonymous_output_11 n/a
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_anonymous_output_12 #main/_anonymous_output_12 n/a
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_anonymous_output_13 #main/_anonymous_output_13 n/a
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_anonymous_output_14 #main/_anonymous_output_14 n/a
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_anonymous_output_15 #main/_anonymous_output_15 n/a
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_anonymous_output_16 #main/_anonymous_output_16 n/a
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_anonymous_output_17 #main/_anonymous_output_17 n/a
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_anonymous_output_18 #main/_anonymous_output_18 n/a
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_anonymous_output_19 #main/_anonymous_output_19 n/a
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_anonymous_output_2 #main/_anonymous_output_2 n/a
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_anonymous_output_3 #main/_anonymous_output_3 n/a
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_anonymous_output_4 #main/_anonymous_output_4 n/a
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_anonymous_output_5 #main/_anonymous_output_5 n/a
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_anonymous_output_6 #main/_anonymous_output_6 n/a
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_anonymous_output_7 #main/_anonymous_output_7 n/a
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_anonymous_output_8 #main/_anonymous_output_8 n/a
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_anonymous_output_9 #main/_anonymous_output_9 n/a
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Version History

1 (earliest) Created 22nd Apr 2024 at 11:21 by Helena Rasche

Added/updated 3 files


Open master 828b298
help Creators and Submitter
Creators
  • Wendi Bacon
  • Julia Jakiela
Submitter
Activity

Views: 435   Downloads: 91

Created: 22nd Apr 2024 at 11:21

Last updated: 22nd Apr 2024 at 11:21

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